The xerocomus spadiceus lectin , xsl , was isolated from extracts of fruiting bodies of the mushroom xerocomus spadiceus using a procedure that involved ( nh4 ) 2so4 precipitation , anion exchange chromatography on deae - cellulose , affinity chromatography on affi - gel blue gel , cation exchange chromatography on cm - cellulose , and gel filtration by fast protein liquid chromatography on superdex 75 从砖红绒盖牛肝菌( xerocomusspadiceus )子实体粗提物中,经过deae -纤维素阴离子交换层析、 affi - gelbluegel亲和层析、 cm -纤维素阳离子交换层析和superdex75fplc凝胶过滤,纯化了砖红绒盖牛肝菌凝集素。
Fast protein liquid chromatography (FPLC), is a form of liquid chromatography that is often used to analyze or purify mixtures of proteins. As in other forms of chromatography, separation is possible because the different components of a mixture have different affinities for two materials, a moving fluid (the mobile phase)mobile phase can contain imidazole which passes through a porous solid (the stationary phase).